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mouse anti beta actin  (Proteintech)


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    Structured Review

    Proteintech mouse anti beta actin
    Mouse Anti Beta Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 8235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+%CE%B2+actin+monoclonal+antibody/beta+Actin+Monoclonal+antibody/10__1038_slash_s41419___026___09312___w-367-68-72
    Average 97 stars, based on 8235 article reviews
    mouse anti beta actin - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Membrane:

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). .. After washing, the membrane was incubated with horseradish peroxidase-labeled secondary antibodies at room temperature for 1 h. Finally, the membrane was developed using an enhanced chemiluminescence reagent (Invitrogen, California, USA), and the signals were detected and analyzed using the BIO-RAD ChemiDoc MP system.

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair.
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4°C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). ..

    Saline:

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). .. After washing, the membrane was incubated with horseradish peroxidase-labeled secondary antibodies at room temperature for 1 h. Finally, the membrane was developed using an enhanced chemiluminescence reagent (Invitrogen, California, USA), and the signals were detected and analyzed using the BIO-RAD ChemiDoc MP system.

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair.
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4°C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). ..

    Article Title: TGEV activates RIG-I/IFN-β/STAT1 axis to promote NLRC5-mediated SLA-I upregulation.
    Article Snippet: The ST cell samples were collected and lysed in RIPA buffer (Solarbio, China) containing 1% protease inhibitor cocktail (MCE, China) for 20 min on ice, followed by denaturation at 100°C for 10 min. Electrophoretic fractionation of proteins was carried out on 12% polyacrylamide gels and subsequently transferred onto nitrocellulose membranes. .. The membranes were blocked in 5% skim milk prepared in Tris-buffered saline with Tween-20 (TBST) at 25°C for 2 h, then incubated overnight at 4°Cwith the following primary antibodies: mouse anti-SLA-I monoclonal antibody (Novus Biologicals, USA, 1:400), mouse anti-β-actin monoclonal antibody (proteintech, China, 1:1000), monoclonal antibody against TGEV-N protein (provided by our laboratory), STAT1 monoclonal antibody (Proteintech, China, 1:5000), and RIG-I/DDX58 polyclonal antibody AR TIC LE IN PR ES S (Proteintech, China, 1:1000). ..

    Incubation:

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). .. After washing, the membrane was incubated with horseradish peroxidase-labeled secondary antibodies at room temperature for 1 h. Finally, the membrane was developed using an enhanced chemiluminescence reagent (Invitrogen, California, USA), and the signals were detected and analyzed using the BIO-RAD ChemiDoc MP system.

    Article Title: Functional extracellular vesicles enable injectable hydrogels with stable osteogenic properties for minimally invasive bone defect repair.
    Article Snippet: .. The membrane was blocked with 5% skim milk in tris-buffered saline and incubated overnight at 4°C with primary antibodies, including rabbit anti-CD63 polyclonal antibody (Wanleibio, Shenyang, China), rabbit anti-Alix monoclonal antibody (Clone No: E6P9B, CST, MA, USA), rabbit anti-TSG101 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), rabbit anti-BMP2 polyclonal antibody (Beyotime Biotechnology, Shanghai, China), mouse anti-β-ACTIN monoclonal antibody (Clone No: 2D4H5, Proteintech, Wuhan, China), and rabbit anti-GM130 monoclonal antibody (Clone No: D6B1, CST, MA, USA). ..

    Article Title: Duck IFNγ Restricts Duck Tembusu Virus Replication by Disrupting Viral RNA Synthesis/Translation.
    Article Snippet: .. Protein samples were separated on 12% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad, USA) at 220 mA for 80 min. Membranes were blocked with 5% nonfat dry milk at room temperature for 3 h and then incubated with one of the following primary antibodies: mouse anti-Flag monoclonal antibody (TransGen Biotech, Beijing, China), rabbit anti-NS1 polyclonal antibody (prepared in this study), rabbit anti-NS3 polyclonal antibody (prepared in this study), or mouse anti-β-actin monoclonal antibody (Proteintech, Chicago, IL, USA). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies: goat anti-rabbit IgG (H + L) (Proteintech, USA) or goat anti-mouse IgG (H + L) (Immunoway, San Jose, CA, USA).

    Article Title: TGEV activates RIG-I/IFN-β/STAT1 axis to promote NLRC5-mediated SLA-I upregulation.
    Article Snippet: In separate experiments, uninfected ST cells were treated with recombinant human IFN-β protein (Univ, China) and Anifrolumab (MCE, China) at concentrations of 10, 100, and 1000 ng/mL for 24 h. These samples were lysed in RIPA supplemented with 1% protease inhibitors on ice for 20 min, denatured at 100°C for 10 min, and subjected to electrophoresis on 12% polyacrylamide gels. .. After transfer, membranes were incubated overnight at 4°C with primary antibodies: mouse anti-SLA-I monoclonal antibody (Novus Biologicals, USA, 1:400), mouse anti-β-actin monoclonal antibody (proteintech, China, 1:1000), monoclonal antibody against TGEV-N protein (provided by our laboratory), and the NLRC5 polyclonal antibody provided by our laboratory. .. Following primary antibody incubation, membranes were probed with HRP‐conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Proteintech, China, 1:4000).

    Article Title: TGEV activates RIG-I/IFN-β/STAT1 axis to promote NLRC5-mediated SLA-I upregulation.
    Article Snippet: The ST cell samples were collected and lysed in RIPA buffer (Solarbio, China) containing 1% protease inhibitor cocktail (MCE, China) for 20 min on ice, followed by denaturation at 100°C for 10 min. Electrophoretic fractionation of proteins was carried out on 12% polyacrylamide gels and subsequently transferred onto nitrocellulose membranes. .. The membranes were blocked in 5% skim milk prepared in Tris-buffered saline with Tween-20 (TBST) at 25°C for 2 h, then incubated overnight at 4°Cwith the following primary antibodies: mouse anti-SLA-I monoclonal antibody (Novus Biologicals, USA, 1:400), mouse anti-β-actin monoclonal antibody (proteintech, China, 1:1000), monoclonal antibody against TGEV-N protein (provided by our laboratory), STAT1 monoclonal antibody (Proteintech, China, 1:5000), and RIG-I/DDX58 polyclonal antibody AR TIC LE IN PR ES S (Proteintech, China, 1:1000). ..

    SDS Page:

    Article Title: Duck IFNγ Restricts Duck Tembusu Virus Replication by Disrupting Viral RNA Synthesis/Translation.
    Article Snippet: .. Protein samples were separated on 12% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad, USA) at 220 mA for 80 min. Membranes were blocked with 5% nonfat dry milk at room temperature for 3 h and then incubated with one of the following primary antibodies: mouse anti-Flag monoclonal antibody (TransGen Biotech, Beijing, China), rabbit anti-NS1 polyclonal antibody (prepared in this study), rabbit anti-NS3 polyclonal antibody (prepared in this study), or mouse anti-β-actin monoclonal antibody (Proteintech, Chicago, IL, USA). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies: goat anti-rabbit IgG (H + L) (Proteintech, USA) or goat anti-mouse IgG (H + L) (Immunoway, San Jose, CA, USA).

    Synthesized:

    Article Title: hnRNPA2B1 induces HBV cccDNA degradation by recruiting APOBEC3B.
    Article Snippet: The pHBV1.3 and pHBV1.3-mer X-null expressing plasmids were purchased f s ( A R c b A C ( a m m p a F b s s o M B 8 T a e w G E c T p i g i s t r p l 2 s a H c p o m p H H t c p u a o T m D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag149/8496867 by U niversidade Federal de Santa C atarina (U FSC ) user on 26 February 2026 rom Addgene (USA). .. The small interfering RNAs (siRNAs) pecific for APOBEC3B were synthesized from GenePharma Shanghai, China) ( Supplementary Table S3 ). ntibodies and reagents abbit anti-hnRNPA2B1 polyclonal antibody (A1162, Ablonal, Wuhan, China), rabbit anti-HBc polyclonal antiody (B0586, Dako, Copenhagen, Denmark), rabbit antiPOBEC3B polyclonal antibody (14559-1-AP, Proteintech, hicago, IL, USA), mouse anti- β-actin monoclonal antibody 66009-1Ig, Proteintech), mouse anti-FLAG-tag monoclonal ntibody (M185-3, MBL, Nagoya, Japan), mouse anti-HA onoclonal antibody (M180-3, MBL), mouse anti-ubiquitin onoclonal antibody (sc-8017, Santa), and rabbit anti-HBx olyclonal antibody (ab39716, Abcam). .. The streptavidin dynbeads were purchased from Invitrogen (11205D, Thermo isher Scientific, Waltham, MA, USA).



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